ISSN 2636-2937
 

Original Research (Original Article) 
JVS. 2019; 5(1): 124-133
doi: 10536


Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon

Riham Hassan Bassam, Hussein A. Hussein, Mohamed M. Amer, Ahmed A. El-Sanousi.

Abstract
Background: Since the first report concerning the existence of avian influenza subtype H9N2 (AI H9N2) in 2006 in Lebanon; a lot of pathological changes and mortalities were seen among chicken flocks suggesting the influence of the AI H9N2 virus.
Objectives this study reports the circulation of H9N2 associated with mortalities in broiler sector and decrease egg production in layer hens and breeders in Lebanon.
Methods: A total of 22 poultry flocks have been tested by real-time reverse transcription polymerase chain reaction (rt-RT-PCR) for the presence of H9N2 in different types of chickens including broilers, breeders, laying hens, backyard chickens and ducks. Tracheal swabs were collected from flocks raised in different governorates in Lebanon.
Results: Testing of samples with rt-RT-PCR revealed that 6 flocks reared in 4 Lebanese governorates were positive (CT ranged from 20.42 to 33.86). A Positive sample with CT 20.42 was tested and partially sequenced and submitted to GenBank, acquired accessions number MH266696 and designated A/chicken/Lebanon/21-E03/2018 (H9N2). Phylogenetic analysis of the obtained sequence cluster the characterized strain along with (formerly) G1-like viruses’ subtype B like those circulating in the Middle East.
Conclusion: the AI H9N2 avian influenza virus is circulating causing major problem among chickens. More studies need to be addressed to understand the real effect of the H9 virus on chickens and humans in Lebanon.

Key words: Avian Influenza, H9N2, ELISA, Real-time PCR, chicken, Lebanon.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Riham Hassan Bassam
Articles by Hussein A. Hussein
Articles by Mohamed M. Amer
Articles by Ahmed A. El-Sanousi
on Google
on Google Scholar
Article Statistics
 Viewed: 1466
Downloaded: 43
Cited: 0


How to Cite this Article
Pubmed Style

Bassam RH, Hussein HA, Amer MM, El-Sanousi AA. Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. JVS. 2019; 5(1): 124-133. doi:10536


Web Style

Bassam RH, Hussein HA, Amer MM, El-Sanousi AA. Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. http://www.j-virol-sci.com/?mno=10536 [Access: November 11, 2019]. doi:10536


AMA (American Medical Association) Style

Bassam RH, Hussein HA, Amer MM, El-Sanousi AA. Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. JVS. 2019; 5(1): 124-133. doi:10536



Vancouver/ICMJE Style

Bassam RH, Hussein HA, Amer MM, El-Sanousi AA. Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. JVS. (2019), [cited November 11, 2019]; 5(1): 124-133. doi:10536



Harvard Style

Bassam, R. H., Hussein, . H. A., Amer, . M. M. & El-Sanousi, . A. A. (2019) Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. JVS, 5 (1), 124-133. doi:10536



Turabian Style

Bassam, Riham Hassan, Hussein A. Hussein, Mohamed M. Amer, and Ahmed A. El-Sanousi. 2019. Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. Journal of Virological Sciences, 5 (1), 124-133. doi:10536



Chicago Style

Bassam, Riham Hassan, Hussein A. Hussein, Mohamed M. Amer, and Ahmed A. El-Sanousi. "Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon." Journal of Virological Sciences 5 (2019), 124-133. doi:10536



MLA (The Modern Language Association) Style

Bassam, Riham Hassan, Hussein A. Hussein, Mohamed M. Amer, and Ahmed A. El-Sanousi. "Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon." Journal of Virological Sciences 5.1 (2019), 124-133. Print. doi:10536



APA (American Psychological Association) Style

Bassam, R. H., Hussein, . H. A., Amer, . M. M. & El-Sanousi, . A. A. (2019) Serosurveillance and Molecular Characterization of H9N2 Avian Influenza Virus in Lebanon. Journal of Virological Sciences, 5 (1), 124-133. doi:10536





Most Viewed Articles
  • Co-circulation of three different serotypes of FMD virus in Egypt
    Mahmoud talaat attia, Emad foad Elgendy, Momtaz Abdelhady Shahein, Samy Gomaa Kasem, madiha salah ibrahim
    JVS. 2017; 2(1): 102-113
    » Abstract

  • Isolation of H5 Highly Pathogenic Avian Influenza Virus from Cattle Egret (Bubulcus ibis) near Affected Broiler Chicken Flocks in Egypt
    Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef
    JVS. 2017; 2(1): 62-70
    » Abstract

  • Identification and Characterization of Soybean vein necrosis virus (SVNV): A newly Isolated Thrips-Borne Tospovirus in Egypt
    Abeer Salah Abd El-Wahab, Manal Ali El-Shazly
    JVS. 2017; 1(Vol. 1, Jan. 2017): 76-90
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli
    Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila
    JVS. 2017; 1(Vol. 1, Jan. 2017): 100-113
    » Abstract

  • Herpes Simplex virus type 1 persistence in water from different sources
    Dina Nadeem Abd-Elshafy, Mahmoud Mohamed Bahgat
    JVS. 2017; 2(1): 114-123
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt
    Soha Ibrahim Mohamed, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, Sayed Ahmed Salem
    JVS. 2017; 2(1): 1-9
    » Abstract

  • first isolation and identification of EHV-4 causing abortion in Egypt
    Ahmed Fawzy Afify, Basem M. Ahmed, Sayed Ahmed Salem, Ahmed Abd El-Ghani El-Sanousi, Mohamed Abd El-Hameed Shalaby
    JVS. 2017; 2(1): 92-101
    » Abstract

  • Most Downloaded
  • Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine
    ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi
    JVS. 2017; 1(Vol. 1, Jan. 2017): 27-39
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Very virulent IBDV strain Egypt/IBDV/Behera2011: Macroscopic and microscopic lesions accompanied with induced mortalities in SPF-chicks
    Reem A. Suliman, Basem M. Ahmed, Mounir M. El-Safty, Hussein Aly Hussein
    JVS. 2017; 2(1): 79-91
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • Identification and Molecular Characterization of a Phytoplasma Associated With Lemon Witches’-Broom in Egypt
    Rasha M. Mahrous, Ahmed K. El-Attar, Ahlam A. ALWatban, Sohair I. EL-Afifi, Nagwa M. Aref
    JVS. 2018; 3(1): 30-41
    » Abstract » doi: 283423

  • Genetic and Antigenic Characterization of Infectious Bronchitis Virus Strains in Egypt
    Sara Mahmoud Ameen, Abdullah Selim, Ahmed El-Sanousi, Mohamed Shalaby
    JVS. 2017; 2(1): 42-55
    » Abstract

  • Genetic characterization of rabbit hemorrhagic disease virus from naturally-infected rabbits in Sharkia governorate, Egypt
    Naglaa F.S. Awad, Gamelat K.F. Kotb
    JVS. 2018; 3(1): 10-19
    » Abstract » doi: 282147

  • Molecular characterization of foot and mouth disease virus (O-EA3) isolated during 2016 outbreak in Egypt
    Muhammad Gamal Khodary, Ayman Hanea El-Deeb, mohamed maged Emara, Othman El mahdy Othman, Hussein Ali Hussein
    JVS. 2018; 3(1): 1-9
    » Abstract » doi: 286803

  • Molecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate.
    Ashraf Shabaan Khameis, lamya Fathi Atteya, Ayman Hmid Mansour, Heba Ahmad Abdelhady, Ashraf Ahmad Saad
    JVS. 2018; 3(1): 49-57
    » Abstract » doi: 276464

  • Most Cited Articles