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Original Article 
JVS. 2019; 5(1): 76-85
doi: 26749


Detection of Potato Virus M in commercial local potato seed tubers in Egypt

Allam Arafat Megahed, Badawi Abdelsalam Othman , Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin .

Abstract
Background: Potato virus M (PVM) is found in potato cultivars worldwide including Egypt. Potato tubers are a common source of the virus during host cultivations. The yield loss can range from 15% to 45% and heavy losses can occur upon mixed infection with PVM and PVS or PVX.
Methods: The PVM was detected in symptomatic infected potato plants during growing seasons for the cultivated commercial local tuber seeds using Double Antibody Sandwich-Enzyme Linked Immuno-Sorbent Assay (DAS-ELISA). The PVM was mechanically isolated on potato cv. Diamant. Host range was carried out by mechanical inoculation of PVM on a set of different plant species. Electron Microscopy was used to examine the virus morphology. The cytopathic effect of the virus on tissue ultrathin section was examined by transmission electron microscope (TEM). Finally, the PVM isolate was detected using reverse transcription-polymerase chain reaction (RT-PCR) in the sprouts after dormancy breaking for the harvested potato tubers.
Results: The obtained results demonstrated that, PVM isolate causes severe symptoms on potato plants under the greenhouse conditions. The infected potato cv. Diamant gave positive DAS-ELISA result using polyclonal antibodies against PVM. Moreover, the RT-PCR confirmed PVM in tuber sprouts using coat protein gene specific primers with an expected amplicon of 520 bp. Electron Microscopy examination revealed that, the viral particles were slightly flexuous rod shape with about 680 x 13 nm in size. Ultrastructure analysis showed different degrees of chloroplast degradation in PVM-infected potato leaf tissues.
Conclusion: Occurrence of PVM was detected and confirmed in commercial potato seed tubers in Egypt using the RT-PCR technique.

Key words: Potato Virus M; potato seeds; DAS-ELISA; RT-PCR; Virus morphology; Cytopathology


 
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How to Cite this Article
Pubmed Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS. 2019; 5(1): 76-85. doi:26749


Web Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. http://www.j-virol-sci.com/?mno=26749 [Access: January 29, 2020]. doi:26749


AMA (American Medical Association) Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS. 2019; 5(1): 76-85. doi:26749



Vancouver/ICMJE Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS. (2019), [cited January 29, 2020]; 5(1): 76-85. doi:26749



Harvard Style

Megahed, A. A., , B. A. O., Masry, S. S. E., Faiesal, A. A., & (2019) Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS, 5 (1), 76-85. doi:26749



Turabian Style

Megahed, Allam Arafat, Badawi Abdelsalam Othman, Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin, and . 2019. Detection of Potato Virus M in commercial local potato seed tubers in Egypt. Journal of Virological Sciences, 5 (1), 76-85. doi:26749



Chicago Style

Megahed, Allam Arafat, Badawi Abdelsalam Othman, Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin, and . "Detection of Potato Virus M in commercial local potato seed tubers in Egypt." Journal of Virological Sciences 5 (2019), 76-85. doi:26749



MLA (The Modern Language Association) Style

Megahed, Allam Arafat, Badawi Abdelsalam Othman, Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin, and . "Detection of Potato Virus M in commercial local potato seed tubers in Egypt." Journal of Virological Sciences 5.1 (2019), 76-85. Print. doi:26749



APA (American Psychological Association) Style

Megahed, A. A., , B. A. O., Masry, S. S. E., Faiesal, A. A., & (2019) Detection of Potato Virus M in commercial local potato seed tubers in Egypt. Journal of Virological Sciences, 5 (1), 76-85. doi:26749








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