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Original Article 


Molecular Diagnosis of Avian Influenza Virus from Different Avian Species

Mohamed Fekry ElKersh , Fatma Mohamed Abdalla , Gamilat Kotb Farg, Soad A. Nasef, Ahmed AbdelSamiee Ali.

Abstract
Avian Influenza Virus (AIV) was isolated from Egypt in 2006, from this year AIV has a great concern on poultry production, due to the high mutation rate of AIV that leads to generating of new generation of the virus, so that the molecular diagnosis of AIV from different avian species should be done regularly. We collected 122 sample representing 12 different area in Egypt and seven different species of poultry produced and traded in Egypt. These samples were isolated on SPF eggs, detected for AIV infection Via HA, RT-PCR and gene sequencing. We analyzed the sequence on gene bank and using the needed programs to detect the relationship between the new isolates and the AIV isolates presented in Egypt since 2006. Where 35 and 55 samples showed positive result for H5 and H9 respectively.

Key words: Avian Influenza, PCR, Sequencing


 
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How to Cite this Article
Pubmed Style

MFE, FMA, Farg GK, Nasef SA, Ali AA, . Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. JVS. 2019; 6(1): 1-11.


Web Style

MFE, FMA, Farg GK, Nasef SA, Ali AA, . Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. http://www.j-virol-sci.com/?mno=27045 [Access: January 27, 2020].


AMA (American Medical Association) Style

MFE, FMA, Farg GK, Nasef SA, Ali AA, . Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. JVS. 2019; 6(1): 1-11.



Vancouver/ICMJE Style

MFE, FMA, Farg GK, Nasef SA, Ali AA, . Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. JVS. (2019), [cited January 27, 2020]; 6(1): 1-11.



Harvard Style

, M. F. E. . ., , F. M. A., Farg, G. K., Nasef, S. A., Ali, A. A. & (2019) Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. JVS, 6 (1), 1-11.



Turabian Style

, Mohamed Fekry ElKersh , Fatma Mohamed Abdalla, Gamilat Kotb Farg, Soad A. Nasef, Ahmed AbdelSamiee Ali, and . 2019. Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. Journal of Virological Sciences, 6 (1), 1-11.



Chicago Style

, Mohamed Fekry ElKersh , Fatma Mohamed Abdalla, Gamilat Kotb Farg, Soad A. Nasef, Ahmed AbdelSamiee Ali, and . "Molecular Diagnosis of Avian Influenza Virus from Different Avian Species." Journal of Virological Sciences 6 (2019), 1-11.



MLA (The Modern Language Association) Style

, Mohamed Fekry ElKersh , Fatma Mohamed Abdalla, Gamilat Kotb Farg, Soad A. Nasef, Ahmed AbdelSamiee Ali, and . "Molecular Diagnosis of Avian Influenza Virus from Different Avian Species." Journal of Virological Sciences 6.1 (2019), 1-11. Print.



APA (American Psychological Association) Style

, M. F. E. . ., , F. M. A., Farg, G. K., Nasef, S. A., Ali, A. A. & (2019) Molecular Diagnosis of Avian Influenza Virus from Different Avian Species. Journal of Virological Sciences, 6 (1), 1-11.








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