Journal of Virological Sciences Service by

Home Journal Issues Guide for Authors Editorial Board About Journal Aims & Scope


Original Article 
JVS. 2018; 3(1): 42-48
doi: 277656


Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus

Ahmed Abdelhaleem Azab, Wesam Hasan Mady, Ali Zanaty, Mahmoud Samir.

Abstract
Infectious bronchitis virus is an acute contagious disease cause severe economic losses in poultry production worldwide. Virus isolation is the golden standard for IBV diagnosis so the aim of this study was comparing the different biological systems for isolation of infectious bronchitis virus. The Egyptian varian2 IBV strain representative to the current circulating strain in Egypt was isolated in SPF-ECE, chicken embryo kidney cell (CEK) and in vero cell line and was confirmed for IBV detection by Reverse transcriptase Real time PCR (RRT-PCR) using IB specific primers. Comparison of different isolation methods was done by generating a standard curve by real time PCR. The results revealed that The propagated virus in CEK showed the highest virus titer (5.952 x 106 and 3.245 x 106) than vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x 105) after both 48 and 72 hours respectively.

Key words: Infectious bronchitis virus, Chicken Embryo Kidney (CEK), Vero cell, isolation, standard curve, Embryonated Chicken Egg (ECE)


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Ahmed Abdelhaleem Azab
Articles by Wesam Hasan Mady
Articles by Ali Zanaty
Articles by Mahmoud Samir
on Google
on Google Scholar
Article Statistics
 Viewed: 155
Downloaded: 41
Cited: 0


How to Cite this Article
Pubmed Style

Azab AA, Mady WH, Zanaty A, Samir M. Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. JVS. 2018; 3(1): 42-48. doi:277656


Web Style

Azab AA, Mady WH, Zanaty A, Samir M. Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. http://www.j-virol-sci.com/?mno=277656 [Access: July 16, 2018]. doi:277656


AMA (American Medical Association) Style

Azab AA, Mady WH, Zanaty A, Samir M. Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. JVS. 2018; 3(1): 42-48. doi:277656



Vancouver/ICMJE Style

Azab AA, Mady WH, Zanaty A, Samir M. Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. JVS. (2018), [cited July 16, 2018]; 3(1): 42-48. doi:277656



Harvard Style

Azab, A. A., Mady, W. H., Zanaty, A. & Samir, M. (2018) Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. JVS, 3 (1), 42-48. doi:277656



Turabian Style

Azab, Ahmed Abdelhaleem, Wesam Hasan Mady, Ali Zanaty, and Mahmoud Samir. 2018. Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. Journal of Virological Sciences, 3 (1), 42-48. doi:277656



Chicago Style

Azab, Ahmed Abdelhaleem, Wesam Hasan Mady, Ali Zanaty, and Mahmoud Samir. "Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus." Journal of Virological Sciences 3 (2018), 42-48. doi:277656



MLA (The Modern Language Association) Style

Azab, Ahmed Abdelhaleem, Wesam Hasan Mady, Ali Zanaty, and Mahmoud Samir. "Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus." Journal of Virological Sciences 3.1 (2018), 42-48. Print. doi:277656



APA (American Psychological Association) Style

Azab, A. A., Mady, W. H., Zanaty, A. & Samir, M. (2018) Different culturing systems of IBV-Eg-F170-2015 strain of Egypt variant 2 infectious bronchitis virus. Journal of Virological Sciences, 3 (1), 42-48. doi:277656



AUTHOR LOGIN

REVIEWER LOGIN

Indexed In



The articles in Journal of Virological Sciences are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.