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Original Article 
JVS. 2018; 4(1): 24-32


Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission

Shimaa M.G. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A.M. Eid.

Abstract
Background: Avian Reovirus (ARV) is involved in many pathologic syndromes of chicken including the both major syndromes, Viral Arthritis (VA) and Runting Stunting Syndrome (RSS), which have great negative impacts on the poultry industry. Although ARV was reported in Egypt since 1984 but only few studies figured out the implication of ARV only in the uneven growth of some Egyptian chicken flocks.
Aim: Clinical and molecular investigation of avian reovirus infection in broiler chickens with availability of Vertical Transmission
Methods: Herein, 18 chickens derived from 4 broiler flocks within Sharkia Province, Egypt suffering from different degrees of lameness and/or stunting were clinically and molecularly examined for the presence of ARV.
Results: By clinical examination, one third (n=6) of examined chickens showed bilateral arthritis with tendons included microscopic changes consistent with the typical ARV infection. Additionally, 8 chickens (44.4%) showed stunting and another one chicken (5.6%) showed poor feathering while the last 3 chickens (16.67%) appeared diarrheic. All clinically affected chickens showed varied postmortem lesions that almost associated with ARV infections. Further molecular detection of ARV within the clinically affected samples (n=18) by RT-PCR using a specific primer set targeting a conserved sequence within ARV- sigma C protein revealed 7 positive samples (38.9%). All positive samples (n=7) were successfully isolated on specific pathogen free emryonated chicken eggs (SPF-ECE). Additional RT-PCR testing and re-isolation of ARV from ECEs of a breeder flock of a history of uneven growth and/or arthritis revealed ARV infection in 10% (n=6) of examined ECEs.
Conclusion: These results indicated the incrimination of ARV infection in some arthritis infections beside the low food conversion rate of chickens within Sharkia Province, Egypt. Moreover, the obtained data highlight the importance of vertical transmission in the field spread of ARV infections.

Key words: ARVs, Isolation, Transovarial transmission, Arthritis, Malabsorption


 
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How to Cite this Article
Pubmed Style

Mansour SM, ElBakrey RM, Orabi A, Ali H, Eid AA. Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. JVS. 2018; 4(1): 24-32.


Web Style

Mansour SM, ElBakrey RM, Orabi A, Ali H, Eid AA. Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. http://www.j-virol-sci.com/?mno=300980 [Access: March 22, 2019].


AMA (American Medical Association) Style

Mansour SM, ElBakrey RM, Orabi A, Ali H, Eid AA. Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. JVS. 2018; 4(1): 24-32.



Vancouver/ICMJE Style

Mansour SM, ElBakrey RM, Orabi A, Ali H, Eid AA. Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. JVS. (2018), [cited March 22, 2019]; 4(1): 24-32.



Harvard Style

Mansour, S. M., ElBakrey, . R. M., Orabi, . A., Ali, . H. & Eid, . A. A. (2018) Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. JVS, 4 (1), 24-32.



Turabian Style

Mansour, Shimaa M.G., Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, and Amal A.M. Eid. 2018. Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. Journal of Virological Sciences, 4 (1), 24-32.



Chicago Style

Mansour, Shimaa M.G., Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, and Amal A.M. Eid. "Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission." Journal of Virological Sciences 4 (2018), 24-32.



MLA (The Modern Language Association) Style

Mansour, Shimaa M.G., Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, and Amal A.M. Eid. "Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission." Journal of Virological Sciences 4.1 (2018), 24-32. Print.



APA (American Psychological Association) Style

Mansour, S. M., ElBakrey, . R. M., Orabi, . A., Ali, . H. & Eid, . A. A. (2018) Isolation and Detection of Avian Reovirus from Tenosynovitis and Malabsorption Affected Broiler Chickens with Involvement of Vertical Transmission. Journal of Virological Sciences, 4 (1), 24-32.



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