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Original Article 
doi: 556


Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017

Akram Ibrahim Aboelkhair, Ayman Hany El-Deeb, Momtaz Abdelhady Shahin, Hussein Ali Hussein.

Abstract
Lumpy skin disease is an infectious viral disease caused by Lumpy skin disease virus (LSDV) affecting cattle causing skin lesions. It`s a member of Poxviridea family, genus Capri-pox, and closely related to sheep and goat pox. In Egypt, the protection from lumpy skin disease (LSD) among cattle population was carried out using sheep poxvirus vaccine. last year ( 2016-2017) revealed many cases of LSD in sheep pox vaccinated cattle in Egypt . In this study LSDV was isolated from sheep pox vaccinated cattle showing LSD signs to confirm LSD viral infection even in sheep pox vaccinated cattle . 130 Nodular samples were collected from 150 vaccinated cattle showing signs of LSD,Then Molecular detection of LSDV with PCR using G-protein-coupled chemokine receptor (G-PCR) gene primer revealed positive result for LSDV, So classical Isolation of LSDV was started by inoculation of nodules on Chorioallantoic membrane (CAM) 9-11 day old SPF eggs, then adaptation on Madin-Derby bovine kidney cell line ( MDBK ); confirmation of LSD isolated virus was done by using Electron microscopy (EM) on suspension of cell culture passage of the virus, Serological identification was done by serum neutralization test ( SNT ) using reference antisera which confirm presence of LSDV . By nucleotide sequences and genetic characterization was conducted on (G-PCR) gene segment of these isolates.

Key words: LSDV; MDBK; CAM; SNT; IFAT; SPV; PCR; Egypt


 
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How to Cite this Article
Pubmed Style

Aboelkhair AI, El-Deeb AH, Shahin MA, Hussein HA. Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. JVS. 2019; 5(1): 1-10. doi:556


Web Style

Aboelkhair AI, El-Deeb AH, Shahin MA, Hussein HA. Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. http://www.j-virol-sci.com/?mno=556 [Access: January 27, 2020]. doi:556


AMA (American Medical Association) Style

Aboelkhair AI, El-Deeb AH, Shahin MA, Hussein HA. Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. JVS. 2019; 5(1): 1-10. doi:556



Vancouver/ICMJE Style

Aboelkhair AI, El-Deeb AH, Shahin MA, Hussein HA. Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. JVS. (2019), [cited January 27, 2020]; 5(1): 1-10. doi:556



Harvard Style

Aboelkhair, A. I., El-Deeb, . A. H., Shahin, . M. A. & Hussein, . H. A. (2019) Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. JVS, 5 (1), 1-10. doi:556



Turabian Style

Aboelkhair, Akram Ibrahim, Ayman Hany El-Deeb, Momtaz Abdelhady Shahin, and Hussein Ali Hussein. 2019. Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. Journal of Virological Sciences, 5 (1), 1-10. doi:556



Chicago Style

Aboelkhair, Akram Ibrahim, Ayman Hany El-Deeb, Momtaz Abdelhady Shahin, and Hussein Ali Hussein. "Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017." Journal of Virological Sciences 5 (2019), 1-10. doi:556



MLA (The Modern Language Association) Style

Aboelkhair, Akram Ibrahim, Ayman Hany El-Deeb, Momtaz Abdelhady Shahin, and Hussein Ali Hussein. "Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017." Journal of Virological Sciences 5.1 (2019), 1-10. Print. doi:556



APA (American Psychological Association) Style

Aboelkhair, A. I., El-Deeb, . A. H., Shahin, . M. A. & Hussein, . H. A. (2019) Lumpy skin disease is circulating ‎among sheep pox vaccinated cattle in ‎Egypt 2017. Journal of Virological Sciences, 5 (1), 1-10. doi:556








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