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Original Article 
JVS. 2019; 5(1): 22-34
doi: 9141


Molecular and pathological study on Chicken Anemia Virus

Hanaa Awad Elsamadony, Laila Abdelghany Tantawy, Sabry Elsayed Omar, Heba Ali Abd Alah.

Abstract
Background: Chicken Anemia Virus is an infectious disease that is causing major economic losses in the poultry industry and these losses are attributed to high mortalities, reduced production and cost for preventive medications.
Objectives: The aim of this study was to identify CAV obtained from farms with problems associated with decreased body weight and immunosuppression. Also to determine their relationship with vaccine used in the field and other reference strains. Then to observe microscopic lesions and to detect CAV antigens by immunohistochemically in thymus and bone marrow tissues of SPF chicks infected with CAV.
Methods: In this study, 30 layer farms of age 40 to 100-days-old from different governorates (Alexandria, Gharbia, Giza and Qalubia) were examined for CAV with real-time PCR and histopathological examination. The positive samples were prepared and inoculated in SPF chicks' one-day-old then at aged 10-days post-inoculation collected organs for PCR, sequencing and immunohistochemically examination.
Results: There's one positive farm of age 100-days-old from Qalubia governorate. The histopathological examination of positive sample showing severe depletion of hematopoietic cells in bone marrow replaced by adipocytes. Spleen exhibited depletion of lymphocytes with focal coagulative necrosis in addition to congested blood vessels. PCR was done on the positive real-time PCR sample, which separate amplified band at 418 bp then the sequences was submitted to the GenBank under accession number MH260568 for the VP1 gene. Our strain has 99% identity with nucleotides of Indian and Japan strains, 97% amino acid identity with the same strains. Our circulating strain has low nucleotides identity 37%, 38% and amino acid identity 15% for both with vaccinal strains (Cux-1N/Germany and CAV/NobilisŪP4) respectively. The high percentage of similarity of nucleotides identity with Egyptian isolates and our strain were with Egypt I/Giza 2009, Egypt II/Fayoum I, Egypt III/Fayoum II that was 86% for all, as well as amino acid identity was 86%, 87% and 86% respectively.
Conclusion: The obtained results indicate that our circulating strain has great differences from vaccinal strains which used in the field, so we need periodic characterization of circulating CAVs in Egypt to improve methods of virus control and to usually determine the relationship of circulating CAV with vaccine strains and other CAV strains. The most obvious thing in this study, that’s we need to make our vaccine from our strain which circulating in the field with periodic updating.

Key words: Chicken anemia virus, SPF chicks, real-time PCR, PCR, histopathology, IIFT and Immune peroxidase.


 
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How to Cite this Article
Pubmed Style

Elsamadony HA, Tantawy LA, Omar SE, Alah HAA, . Molecular and pathological study on Chicken Anemia Virus. JVS. 2019; 5(1): 22-34. doi:9141


Web Style

Elsamadony HA, Tantawy LA, Omar SE, Alah HAA, . Molecular and pathological study on Chicken Anemia Virus. http://www.j-virol-sci.com/?mno=9141 [Access: January 29, 2020]. doi:9141


AMA (American Medical Association) Style

Elsamadony HA, Tantawy LA, Omar SE, Alah HAA, . Molecular and pathological study on Chicken Anemia Virus. JVS. 2019; 5(1): 22-34. doi:9141



Vancouver/ICMJE Style

Elsamadony HA, Tantawy LA, Omar SE, Alah HAA, . Molecular and pathological study on Chicken Anemia Virus. JVS. (2019), [cited January 29, 2020]; 5(1): 22-34. doi:9141



Harvard Style

Elsamadony, H. A., Tantawy, . L. A., Omar, . S. E., Alah, . H. A. A. & (2019) Molecular and pathological study on Chicken Anemia Virus. JVS, 5 (1), 22-34. doi:9141



Turabian Style

Elsamadony, Hanaa Awad, Laila Abdelghany Tantawy, Sabry Elsayed Omar, Heba Ali Abd Alah, and . 2019. Molecular and pathological study on Chicken Anemia Virus. Journal of Virological Sciences, 5 (1), 22-34. doi:9141



Chicago Style

Elsamadony, Hanaa Awad, Laila Abdelghany Tantawy, Sabry Elsayed Omar, Heba Ali Abd Alah, and . "Molecular and pathological study on Chicken Anemia Virus." Journal of Virological Sciences 5 (2019), 22-34. doi:9141



MLA (The Modern Language Association) Style

Elsamadony, Hanaa Awad, Laila Abdelghany Tantawy, Sabry Elsayed Omar, Heba Ali Abd Alah, and . "Molecular and pathological study on Chicken Anemia Virus." Journal of Virological Sciences 5.1 (2019), 22-34. Print. doi:9141



APA (American Psychological Association) Style

Elsamadony, H. A., Tantawy, . L. A., Omar, . S. E., Alah, . H. A. A. & (2019) Molecular and pathological study on Chicken Anemia Virus. Journal of Virological Sciences, 5 (1), 22-34. doi:9141








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