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Original Article 
JVS. 2019; 5(1): 114-123
doi: 9156


Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon

Riham Hassan Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed, Ahmed A. El-Sanousi.

Abstract
Background: IBV is very important disease affecting mainly the respiratory tract of chickens. In Lebanon; there is a few data concerning the IBV among poultry flocks.
Objectives: The aim of the present study is to detect the molecular characterize IBV in samples collected from Lebanese flocks geographically distributed in 7 Governorates.
Methods: Blood sera, tracheal swabs and tissue organs were collected from 60 poultry flocks from different ages, different type of raising which were housed in different Governorates in Lebanon including, broilers, layer hens of breeders, backyard chickens and ducks for serosurveillance and molecular characterization. Also; sequence analysis of the same IBV-positive samples.
Results: flocks had shown high discrepancy of CV% values with regard to ELISA results against IB virus. Out of 60 flocks tested; 12 were positive to the presence of IBV by rt-RT-PCR despite the fact that the detected flocks had a history of previous IBV vaccination except for backyard chickens and ducks. Sequence analysis for three IBV detected viruses had shown 99% similarity with VAR2 serotype present in Egypt.
Conclusion: VAR2-IBV is circulating among chickens demonstrating mortalities which affect poultry industry in Lebanon.

Key words: Key Words: IBV, ELISA, rt-RT-PCR, chicken, Lebanon.


 
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How to Cite this Article
Pubmed Style

Bassam RH, Hussein HA, Amer MM, Ahmed BM, El-Sanousi AA. Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. JVS. 2019; 5(1): 114-123. doi:9156


Web Style

Bassam RH, Hussein HA, Amer MM, Ahmed BM, El-Sanousi AA. Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. http://www.j-virol-sci.com/?mno=9156 [Access: January 29, 2020]. doi:9156


AMA (American Medical Association) Style

Bassam RH, Hussein HA, Amer MM, Ahmed BM, El-Sanousi AA. Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. JVS. 2019; 5(1): 114-123. doi:9156



Vancouver/ICMJE Style

Bassam RH, Hussein HA, Amer MM, Ahmed BM, El-Sanousi AA. Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. JVS. (2019), [cited January 29, 2020]; 5(1): 114-123. doi:9156



Harvard Style

Bassam, R. H., Hussein, . H. A., Amer, . M. M., Ahmed, . B. M. & El-Sanousi, . A. A. (2019) Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. JVS, 5 (1), 114-123. doi:9156



Turabian Style

Bassam, Riham Hassan, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed, and Ahmed A. El-Sanousi. 2019. Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. Journal of Virological Sciences, 5 (1), 114-123. doi:9156



Chicago Style

Bassam, Riham Hassan, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed, and Ahmed A. El-Sanousi. "Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon." Journal of Virological Sciences 5 (2019), 114-123. doi:9156



MLA (The Modern Language Association) Style

Bassam, Riham Hassan, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed, and Ahmed A. El-Sanousi. "Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon." Journal of Virological Sciences 5.1 (2019), 114-123. Print. doi:9156



APA (American Psychological Association) Style

Bassam, R. H., Hussein, . H. A., Amer, . M. M., Ahmed, . B. M. & El-Sanousi, . A. A. (2019) Molecular Characterization of VAR2 among IBV Infected poultry in Lebanon. Journal of Virological Sciences, 5 (1), 114-123. doi:9156








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